A class I ligase ribozyme with reduced Mg2+ dependence: selection, sequence analysis, and tertiary-structure mapping
Published in RNA, 2009
Recommended citation: SC Bagby,1 NH Bergman,1 DM Shechner, C Yen, and DP Bartel (2009). "A class I ligase ribozyme with reduced Mg2+ dependence: selection, sequence analysis, and tertiary-structure mapping." RNA 15:2129. (1joint first authors)
A ribozyme selected for rapid catalysis shows changes to a network of structural interactions that contribute to metal-binding. In vitro evolution, statistics, structural biochemistry.